However, we observed that, similar to the situation described above for prostate cancer cells 22Rv1, stable shRNA down-regulation of miR-145-5p led to an increase in TWIST1 expression in the context of low CPEB1 expression (Late cells), but not in the context of high CPEB1 expression (Early cells) (Figure 3C). This evidence concerns the gene CPEB1 and prostate cancer.