To investigate whether, in CRC cells, the modulation of S1PR2 can also drive the activation of the PI3K/AKT pathway, which is highly expressed in the RKO cell line [27], we quantified the protein levels of the phosphorylated AKT in RKO scramble and RKO S1PR2 OE cells. This evidence concerns the gene S1PR2 and colorectal carcinoma.