To further corroborate the key role of S1PR2 in arresting the expansion of intestinal stem cells, we analyzed in RKO-S1PR2-OE and scramble cells the expression of transcription factor Sex-determining region Y (SRY)-box 9 (SOX9), which is linked to stem cell maintenance [29] and implicated in CRC. The gene discussed is S1PR2; the disease is colorectal carcinoma.