CASP3 and neoplasm: Importantly, IHC staining of tumor tissues revealed that, compared with GW3965 or Afatinib single-agent treatment, a combination of the two agents more significantly inhibited cell growth (decrease of Ki-67 and increase of p27) and induced apoptosis (increase of cleavage caspase-3) (GW3965 or Afatinib vs. GW3965 + Afatinib: P < 0.01; Figure 5E).