Subgroup analysis of pubertal children according to gender revealed no statistically significant difference in serum kisspeptin levels between pubertal children with prediabetes and normal HbA1c ranges in both genders (boys: 58.7 (34.3, 88.0) vs 37.9 (29.7, 70.4) pg/mL, p = 0.055; girls: 50.7 (33.3, 66.9) vs 37.2 (28.7, 63.3) pg/mL, p = 0.146). Here, KISS1 is linked to prediabetes syndrome.