In the non-PID sample (Sample 2), events classified by MG as CD4+ effector memory T-cells and CD4+ naive T-cells had been incorrectly classified by the AG&I module as CD4-CD8- TCRγδ- due to an abnormally low CD4-signal because of a technical (staining) issue (unchecked AG&I plots shown in Figure 2C). The gene discussed is CD8A; the disease is pelvic inflammatory disease.