To explore the mechanism of dysregulated NEAT1 during the early stage of AD, we generated NEAT1-deficient cells (shNEAT1v2 cells) and negative control cells (shCtrl cells) using lentivirus based NEAT1-targeting short hairpin RNA (shRNA) and control shRNA vectors on SH-SY5Y cells. This evidence concerns the gene NEAT1 and Alzheimer disease.