Then we examined hindbrain sections from mice maintained on a HF diet and treated with vehicle, AC3174, and AC170222 alone or in combination (Figure 1E) and processed for RNAscope against c-fos and Calcrl. GLP-1R and CCK1R co-agonism significantly increased neuronal activation in the AP and NTS compared to both mono-agonists, and a majority of the neurons activated by the combinatorial treatment expressed Calcrl. Here, CCKAR is linked to hydrops fetalis.