To figure out whether ASB16-AS1 regulates HuR protein levels through ubiquitination, we treated adrenocortical carcinoma cells with a specific proteasome inhibitor MG132, and the results showed that MG132 treatment abolished the down-regulation of HuR protein expression upon overexpression of ASB16-AS1 (Fig. 7b). The gene discussed is ASB16; the disease is adrenal cortex carcinoma.