We also used an HFD-induced obesity and type 2 diabetes mouse model to assess calorie intake (Supplementary Fig. 8a), blood glucose level (Supplementary Fig. 8b), body size (Supplementary Fig. 8c), cold-induced thermogenesis capacity (Supplementary Fig. 8d), and food intake-related neuropeptide expression (Supplementary Fig. 8e) in WT and Pdk2 KO mice. Here, NPS is linked to obesity due to melanocortin 4 receptor deficiency.