To understand the PDK-mediated metabolic modulation of hypothalamic inflammation and subsequent pathologies in diabetes, we first examined the expression of PDKs (PDK1–4) in the hypothalamus of mice with type 1 diabetes induced by injecting either a single high dose of streptozotocin (STZ) or multiple low doses (MLDS) using quantitative PCR, Western blot analysis, and immunohistochemical analysis. This evidence concerns the gene PDK1 and diabetes mellitus.