PDK2 and diabetes mellitus: To determine the role of PDK2 in hypothalamic astrocytes and neurons in diabetes-induced inflammation and altered feeding behavior, we first generated mice in which the Pdk2 gene was selectively inactivated in hypothalamic astrocytes by bilateral injection of AAV5-GFAP-mCherry-Cre into the hypothalamus of homozygous Pdk2 floxed mice.