To further examine the role of hypothalamic PDK2 in diabetes-induced neuroinflammation and changes in feeding behavior, we assessed the effect of PDK2 overexpression in the Pdk2 KO mice by injecting (icv) PDK2-overexpressing adenovirus (Ad-PDK2-GFP) into the third ventricle (Fig. 6a). Here, PDK2 is linked to diabetes mellitus.