In opposition to these previously documented findings for CPTs, our studies with bladder cancer cells as presented in this report revealed that FL118 has the least ability to inhibit Top1 expression in the resistant cell line HT1376 (Figure 5A), while FL118 has a better ability to inhibit Top1 expression in the FL118-sensitive T24 and UMUC-3 cells (Figure 5B,C). Here, TOP1 is linked to urinary bladder cancer.