We found that all of the three bladder cancer cell lines (HT1376, T24, UMUC-3) have the high constitutive expression and phosphorylation (activation) of AKT (p-Akt on ser473) and ERK1/2 (p-ERK1 on Thr202/Tyr204 and p-ERK2 on Thr185/Tyr187) (Figure 4, Figure S4). Here, AKT1 is linked to urinary bladder cancer.