To detect driver mutations in the TME, we analysed HNSCC-related SNP data and consequently detected 21 putative driver genes, including TP53, TTN, CSMD3, CDKN2A and NOTCH1 (Figure 4A and 4B), which were associated with the TME, using random forest algorithm with 1000 iterations. This evidence concerns the gene CDKN2A and head and neck squamous cell carcinoma.