Pathway analysis of the differentially expressed genes using the STRING protein–protein interaction database identified angiotensin signalling enriched in double-mutant male tumours compared to Ctnnb1 mutant tumours, including Agtr1b, Egr1, Nr4a1 and members of the Fos/Jun family Fos, Fosb and Junb (Fig. 4d and Supplementary Table S4).34 Interestingly, Cyp11b2 expression, a target of this pathway, was not changed in these tumours. This evidence concerns the gene AGTR1 and neoplasm.