In addition, treatment of ARNAX with tumor-ssociated antigen could induce mRNA expression of genes related to cytotoxicity (IFNγ, Gzmb, and Prf1) and chemokines recruiting the CTLs (Cxcl9 and Cxcl10) in tumors, induce effector CTLs infiltration, and facilitate Th1-type anti-tumor immunity in mouse model. This evidence concerns the gene PRF1 and neoplasm.