When they overexpressed miR-34a in mouse myoblast C2C12 cells by lentiviral vector infection and induced miR-34a human primary myotubes by hydrogen peroxide treatment (to simulate oxidative stress), the cells released EVs enriched in miR-34a, which in turn were able to abrogate Sirtuin 1 (sirt1). This evidence concerns the gene SIRT1 and infection.