Although these results are not sufficient to associate the two genes, known to be involved in the development of lymphatic capillaries and valves, with lymphedema, they suggest that it would be worthwhile screening a larger cohort of patients for variants in NRP1 and NRP2, especially in large families with more than one affected subject, and performing a further functional analysis of the variants identified by us in in vitro and in vivo models to confirm their involvement in the development of lymphedema. Here, NRP1 is linked to lymphedema.