We then characterized a subset of adherent cell lines in colony formation assays and find TH5487 causes a concentration-dependent loss of clonogenic potential in the cancer cell lines ACHN and H460, whereas the non-transformed cell lines MRC5 and Ogg1−/− mouse embryonic fibroblasts were unaffected by treatment up to 20 μM (Figure 3D). This evidence concerns the gene OGG1 and cancer.