One previous study reported upregulation of CYP2R1 in the gonadal fat of male mice after 11 weeks on a HFD.(40) We did not observe a similar effect in our study using a 16‐week‐HFD treatment, and did not detect any significant effects of obesity on CYP2R1 expression in mouse WAT. The gene discussed is CYP2R1; the disease is obesity due to melanocortin 4 receptor deficiency.