Interestingly, compared to normal lungs, the quantification of stained cells indicated ROBO2 overexpressed in epithelium at E13.5 and E15.5; bronchi and primordia of bronchiole at E17.5; and in the alveolar duct and mesenchyme at E21.5 (Fig. 3b) in the nitrofen-induced CDH rat model. Here, ROBO2 is linked to congenital diaphragmatic hernia.