USP7 and Familial prostate cancer: To evaluate the expression of USP7 in both normal cells and lung and prostate cancer cells, we performed immunoblotting using samples isolated from hTERT-immortalized retinal pigment epithelial (hTERT-RPE) normal cells, NCI-H460 and A549 NSCLC cells, and LNCaP and DU145 prostate cancer cells (Figure 1a,b).