To model leukemic progression in human SCN, we introduced the CSF3R-d715 mutation by CRISPR-Cas9-mediated genome editing and the RUNX1-D171N mutation by lentiviral transduction (containing IRES-GFP; Figure S1C) in iPSCs from an ELANE mutant-SCN patient (Figures S5A–S5C) and performed RNA sequencing on GFP+ CD34+CD45+ hematopoietic progenitor cells (HPCs) derived from two independent experiments. Here, RUNX1 is linked to severe congenital neutropenia.