YTHDF1 and neoplasm: In vitro experiments showed that YTHDF1 knockdown significantly decreased total HPSCC intracellular iron levels (Figure 2A), whereas in vivo experiments indicated that tumor xenografts with YTHDF1 knockdown had lower ferritin expression, as determined by IHC analysis (Figure 2B). To determine whether YTHDF1 directly promotes HPSCC iron metabolism, we conducted intracellular Fe2+ and ROS analyses.