ST3GAL1 and melanoma: To investigate the role of ST3GAL1 in melanoma progression, we silenced ST3GAL1 in a lung metastatic clone derived from A375 cells (A375 M6)20 and in SSM2c cells using two independent ST3GAL1 shRNAs (LV-shST3GAL1.1 and LV-shST3GAL1.2), which target the 3′-UTR or the CDS region of ST3GAL1 respectively (Fig. 3a).