Moreover, through RNA sequencing, SHC1 (Src homology 2 domain containing transforming protein 1, alias ShcA) was identified as a potential downstream of DEPDC1B, downregulation of which exhibited similar effects on bladder cancer cell functions with DEPDC1B KD and could alleviate the promotion effects of DEPDC1B overexpression on bladder cancer. Here, DEPDC1B is linked to urinary bladder carcinoma.