In this study, combining in vitro and in vivo analyses, we investigated: i) the difference in the expression of TRIB3 in both primary specimens from glioma patients and GBM cell lines and that in controls; ii) the effects of overexpression and knockdown of TRIB3 on GBM cell progression in vitro and in vivo; and iii) the relationship between TRIB3 and autophagic flux in GBM. The gene discussed is TRIB3; the disease is glioblastoma.