To investigate the function of latent genes in drug resistance to sorafenib in HCC, we first detected the mRNA expression of TOP2A, MELK, KIF2C, ASPM, KIF4A, and KIF14 in sorafenib-resistant Huh7-SR and HepG2-SR cells and compared it with that in the corresponding parent cells. Here, ASPM is linked to hepatocellular carcinoma.