To explore the molecular mechanism of KIF14 silencing in reversing acquired resistance to sorafenib in HCC, sorafenib-resistant cells were transfected with control or KIF14 siRNA for 24 h, incubated with 0 or 5 μM sorafenib for another 24 h, and finally subjected to western blotting. The gene discussed is KIF14; the disease is hepatocellular carcinoma.