On the whole, the present study has pictured the mechanisms of HDAC3, TGIF1, TGFβ signaling pathway and miR-296-3p in CRC that knockdown of HDAC3 or TGIF1 or up-regulation of miR-296-3p blocks CRC development and tumor growth via inhibiting TGFβ signaling pathway. The gene discussed is TGFB1; the disease is colorectal carcinoma.