RT-qPCR and western blot analysis were carried out to explain the involved mechanism of HDAC3 in CRC, and findings suggested (Fig. 1a-c, and g, h) that higher expression levels of HDAC3 and TGIF1, and lower expression level of miR-296-3p in CRC tissues. This evidence concerns the gene TGIF1 and colorectal carcinoma.