Subsequently, experiments of HDAC3 and TGIF1 knockdown and miR-296-3p up-regulation are performed to identify their roles in CRC cell progression and it is depicted that either reduction of HDAC3 or TGIF1 or up-regulation of miR-296-3p is suppressive in CRC cell proliferation and metastasis. Here, TGIF1 is linked to colorectal carcinoma.