However, result of AAV genome copies per liver cell (Figure 3Cii; AAV8-HLP-hINSco 7.5 × 109 vg/mouse) and human C-peptide level (Figure 3Dii; AAV8-HLP-hINSco 7.5 × 109 vg/mouse) revealed that mouse 3 contained similar number of AAV genome copies per liver cell and human C-peptide level comparable to mouse 2 suggesting that glucose intolerance in mouse 3 was not due to insufficient expression of human insulin due to fix dose of AAV injection. This evidence concerns the gene INS and Glucose intolerance.