Figure 2 also shows that blood-stage malaria in non-vaccinated mice induces an increase in hepatic mRNA levels of Prf1 with a maximum at early patency on day 4 p.i. Vaccination-protected mice reveal significantly higher expressions of Prf1, not only at maximum expression on day 4 p.i., but also earlier on day 1 p.i. This evidence concerns the gene PRF1 and malaria.