In another clinical study to evaluate the expression of prostate-specific antigen (PSA) which reflect the development of prostate cancer, 43 healthy participants received a supplement containing 40 mg isoflavones (66% daidzein, 24% glycitin, and 10% genistin) and 100 mg curcumin, and 42 volunteers received placebo daily in a double blind study for six months. Here, PROS1 is linked to prostate cancer.