Similar results further established that POLR1B expression was significantly increased in these included CRC cells such as SW480, SW620, HCT116, SW48, CACO2, LOVO, HT29, and RKO cells compared with HIEC cells assayed by qPCR and RT-PCR method (Figure 5D, Supplementary Figure 4B). This evidence concerns the gene POLR1B and colorectal carcinoma.