In order to further ensure the accuracy of two mutations sites, we set more strict filter conditions (set the data filtering standard of 0.001 / 0.0001, as shown in Table 3), and finally determined that only P_101 (exon26:c.A3142G:p.I1048V/exon14:c.G1622A:p.C541Y) and P_136 (exon11:c.G1220A:p.G407D/exon65:c.C8080T:p.R2694X) have two mutations in the FBN1 gene at the same time (According to the incidence rate of 1/10000 in MFS patients, two mutations were confirmed). This evidence concerns the gene FBN1 and Marfan syndrome.