Expanded subclones were screened for reporter readout following infection with HIV-1 ADA5,6, both by direct infection with cell-free HIV stocks and by co-culture with human monocyte-derived macrophages (HMDM) infected 14 days prior with vesicular stomatitis virus g-glycoprotein (VSV-G) pseudotyped ADA (data not shown). This evidence concerns the gene ADA and infection.