When mice were sacrificed 28 days after tumor implantation, those treated with PBS showed diffuse peritoneal metastases and malignant ascites, while those treated with the combination of JX and anti-PD-1 showed suppressed peritoneal tumor dissemination by 98% and, more importantly, eradication of malignant ascites (online supplemental figure S3B–D). Here, PDCD1 is linked to neoplasm.