CD40 and obesity due to melanocortin 4 receptor deficiency: After inducing obesity in C57BL/6 by feeding them a high-fat diet (HFD, 20% kcal carbohydrate, 60% kcal fat, 20% kcal protein) for six consecutive weeks, the animals were treated with the CD40-TRAF6 inhibitor for another six weeks while receiving HFD.