To further confirm that KIAA1199 enhances the proliferation and migration of CCA by upregulating the TGF-β-PI3K-AKT-mediated EMT signaling pathway, we treated QBC939 cells with the small molecule TGF-β inhibitor SB431542 and small molecule PI3K inhibitor LY294002. This evidence concerns the gene TGFB1 and cholangiocarcinoma.