Finally, we also investigated the Reverse Phase Protein Arrays (RPPA) data and confirmed several reported tumor regulating proteins were differentially expressed in obesity group, including 14-3-3β, GTP-binding protein Di-Ras3 (ARHI), Fatty Acid Synthase (FASN) and ribosomal protein S6 (RPS6, Figure 3G). This evidence concerns the gene RPS6 and obesity due to melanocortin 4 receptor deficiency.