ACTA2 and colorectal carcinoma: Consistent with our smFISH data, scRNA-seq data from human CRC tissues20 confirmed that GREM1 expression levels were inversely correlated with ISLR expression (Supplementary Figure 14A) and that GREM1, but not ISLR, transcripts were positively correlated with ACTA2 expression in CRC CAFs (Supplementary Figure 14B).