To evaluate the immuno-wall devices, we analyzed mixed NSCLC cell lysates harboring mutant and WT EGFR. EGFR mutant cells (HCC827 for E746_A750 deletion and H3255 for L858R substitution) and WT (H358) were mixed with 0%, 0.1%, 0.5%, 1%, 5%, and 10% mutant cells. This evidence concerns the gene EGFR and non-small cell lung carcinoma.