Comparison of the myocardial infarct size in mice, production of peroxynitrite, NO and superoxide as well as i-NOS and gp91phox protein expression, it was found that the adiponectin globular domain structure reduced the myocardial ischemia/reperfusion induced i-NOS/gp91phox protein expression, reduced the generation of NO/peroxide, blocked oxygen nitrite formation, and reversed adiponectin−/− mice to expand the infarction effect. This evidence concerns the gene NOS2 and myocardial infarction.