We observed that some uncharacterized phosphorylated protein levels of Ku70 (XRCC6, position Ser520, and Thr455), DNA-PKcs (PRKDC, Ser893, Ser3995 and Ser3205), and PAXX (Ser148) were higher in colon cancer tissues compared to normal colon tissues (Supplementary Figures S1A–K), whereas phosphorylated XLF (NHEJ1, Ser287) was lesser in colon cancer tissues. This evidence concerns the gene PRKDC and malignant colon neoplasm.