In either human colon cancer HT29 cells with endogenously RIP3 expression or HeLa-RIP3 cells with exogenously expressed Flag-tagged RIP3, necroptosis could be induced by TNF plus a pan-caspase inhibitor Z-VAD-FMK (Z-VAD) and Smac-mimic small molecule compound (Smac-mimic). Here, TNF is linked to colonic neoplasm.