When we treated PD models of midbrain-like simBOs with LRRK2 kinase inhibitor, PFE-360, Rab10 phosphorylation was significantly suppressed in both PD- and control-simBOs (Figure 7G) and the LC3B-II levels were rescued in PD-simBOs (Figure 7H), suggesting autophagy are regulated by LRRK2 kinase activity in PD-simBOs. Here, LRRK2 is linked to Parkinson disease.