Since hypoxia has been recognized as an inducer of the activation of the TLR4/MyD88/NF-κB pathway in HCC (Won et al., 2015; Zhang et al., 2016), therefore, we aimed to investigate whether USP13 mediated the hypoxia-induced activation of the TLR4/MyD88/NF-κB pathway in HCC. This evidence concerns the gene TLR4 and hepatocellular carcinoma.