Since TLR4 stabilization is regulated by ubiquitination-mediated degradation (Chuang and Ulevitch, 2004; Lu et al., 2020), TCGA data analysis using the GEPIA web tool (Tang et al., 2017) indicated that the expression of TLR4 mRNA in HCC tissues was significantly lower than that in normal liver tissues (P < 0.0001, Supplementary Figure S4A). This evidence concerns the gene TLR4 and hepatocellular carcinoma.