The result showed a rapid upregulation of IL-1β, IL-6, and TNF-α and infiltration of macrophage in the first 3 days after MI in the peri-infarct/infarct zone, followed by a sharp decline of the expression level of these three cytokines and the number of infiltrated macrophages (Figures 2A,D,G). This evidence concerns the gene TNF and myocardial infarction.