By contrast, the inhibition or knockout of LAT1 expression by inhibitors and small interfering RNA (siRNA) can reduce the migration and invasion of cholangiocarcinoma cells in vitro, suggesting that the transport function of LAT1 is crucial for providing necessary nutrition to metastatic cancer cells (Janpipatkul et al., 2014). This evidence concerns the gene SLC7A5 and cholangiocarcinoma.