Using an approach with great sensitivity to mutant cfDNA fragments at below 1%, GAs were detected in 79% patients. Most frequent GAs were TP53 (35%), VHL (23%), EGFR (17%), NF1 (16%), and ARID1A (12%).Mutations from non-RCC related somatic expansions like CHIP were not excluded55% of variants were of unknown significance45% of SNVs and indels were characterized with known significance. Distribution of GAs amongst patients were as follows: TP53- 30% VHL-32%, NF1-22%, EGFR-13%, and ARID1A-18%. This evidence concerns the gene EGFR and renal cell carcinoma.