AZU1 and nonpapillary renal cell carcinoma: Extracellular vesicles (EVs) directly isolated from surgically resected ccRCC tissues and adjacent normal renal tissues were analyzed with quantitative LC/MS. This analysis identified 3,871 tissue‐exudative EV proteins, among which azurocidin (AZU1) was highly enriched in tumor EVs (fold‐change = 31.59).AZU1 content in EVs was significantly higher in ccRCC patients compared to those from healthy donors.Subsequent functional analyses indicated that EV‐AZU1 could be engaged with vesicle‐mediated hematogenous metastasis of RCC.