Specially, we observed significantly increased mRNA expression level for 10 TBC genes (TBC1D5, TBC1D19, TBC1D13, TBC1D24, TBC1D1, TBC1D16, TBC1D7, TBC1D14, TBC1D10C, and TBC1D22A), and decreased mRNA expression level for 21 TBC genes (TBCK, TBC1D30, TBC1D22B, TBC1D10A, TBC1D17, SGSM1, SGSM2, SGSM3, TBC1D3, TBC1D2, TBC1D2B, TBC1D20, TBC1D9B, TBC1D25, TBC1D21, TBC1D4, TBC1D12, TBC1D15, TBC1D23, TBC1D26, and TBC1D28) in skin cutaneous melanoma (SKCM) compared with normal tissues (Figure 2A). The gene discussed is TBC1D21; the disease is cutaneous melanoma.