To explore the regulatory effects of miR-21 on TPM1, we transfected the miR-21 antisense oligonucleotide (miR-21 inhibitors) and miR-21 oligonucleotide mimics (miR-21 mimics) into the EC109 and EC1 ESCC cell lines. Here, TPM1 is linked to esophageal squamous cell carcinoma.