To demonstrate the specific role of PKM2 in HCC cells, we detected expression of PKM2 in HCC cell lines including HepG2, Hep3B, PLC/RCF/5 (PLC), Huh7, MHCC97-L, MHCC97-H and HCC-LM3 (Supplementary Figure 1A). This evidence concerns the gene HSPG2 and hepatocellular carcinoma.