Furthermore, detection of the representative protein of NF-κB p65 revealed that the expression of NF-κB p65 was significantly up-regulated in colonic tissues of DSS-induced colitis mice as compared to the normal control mice, while HnAb treatment significantly decreased the expression of NF-κB p65 as compared to the vehicle treatment in DSS-induced colitis mice (Figures 5B, C). This evidence concerns the gene NFKB1 and colitis.